Princess Shroob
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Princess Shroob is the ruler of the Shroobs, whom planned to attack the Mushroom World of the past. She plans an invasion, and attacks Toad Town first, infesting the town with purple mushrooms, and capturing nearly everyone in it. She then attacked Princess Peach's Castle and redesigned it to resemble a shroob castle. Her first, and only game is Mario & Luigi: Partners in Time for the Nintendo DS.
| Mario Villains |
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| Major Villains: Donkey Kong | Bowser | Foreman Spike | Wart | Ludwig von Koopa | Lemmy Koopa | Roy Koopa | Iggy Koopa | Wendy O. Koopa | Morton Koopa Jr. | Larry Koopa | Tatanga | Virus | Wario | Kamek | Baby Bowser | Smithy | Big Bob-omb | Waluigi | King Boo | Shadow Mario | Petey Piranha | Bowser Jr. | Cackletta | Fawful | Sir Grodus | Lord Crump | Shadow Queen | Princess Shroob | Count Bleck | Dimentio |
| Minor Villains: Blooper | Bob-omb | Boo | Bullet Bill | Buzzy Beetle | Chain Chomp | Cheep Cheep | Dry Bones | Goomba | Hammer Bros. | Koopa Paratroopa | Koopa Troopa | Lakitu | Magikoopa | Monty Mole | Ninji | Piranha Plant | Pokey | Shroob | Shy Guy | Snifit | Spiny | Thwomp | Whomp | Wiggler |
