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Artwork of Princess Shroob from Mario & Luigi: Partners in Time.
|Series||Mario & Luigi series|
|First game||Mario & Luigi: Partners in Time|
Princess Shroob is the ruler of the Shroobs, who planned to attack the Mushroom World of the past. She plans an invasion, and attacks Toad Town first, infesting the town with purple mushrooms, and capturing nearly everyone in it. She then attacked Princess Peach's Castle and redesigned it to resemble a Shroob Castle. However, the real Princess Shroob is her older sister, who has been trapped in the Cobalt Star by Princess Peach. Then younger Princess Shroob captured Princess Peach and took over. She tries everything to get revenge on her sister instead of staying as leader and letting her rot. Her first, and only game is Mario & Luigi: Partners in Time for the Nintendo DS.
|Major villains||Donkey Kong | Bowser | Foreman Spike | Wart | Ludwig von Koopa | Lemmy Koopa | Roy Koopa | Iggy Koopa | Wendy O. Koopa | Morton Koopa Jr. | Larry Koopa | Tatanga | Virus | Wario | Kamek | Baby Bowser | Smithy | Big Bob-omb | Waluigi | King Boo | Shadow Mario | Petey Piranha | Bowser Jr. | Cackletta | Fawful | Sir Grodus | Lord Crump | Shadow Queen | Princess Shroob | Count Bleck | Dimentio | Pom Pom | Boom Boom|
|Minor villains||Blooper | Bob-omb | Boo | Bullet Bill | Buzzy Beetle | Chain Chomp | Cheep Cheep | Dry Bones | Goomba | Hammer Bros. | Koopa Paratroopa | Koopa Troopa | Lakitu | Magikoopa | Monty Mole | Ninji | Piranha Plant | Pokey | Shroob | Shy Guy | Snifit | Spiny | Thwomp | Whomp | Wiggler|