In the Wario video games, Wario's bike first appeared in Wario Land 4 for the Game Boy Advance. After that game, it appeared regularly in the WarioWare franchise including some of the mini games and the cutscenes.
Super Smash Bros. series
The Wario Bike is also Wario's side special move in Super Smash Bros. Brawl and Super Smash Bros. for 3DS and Wii U. He gets on the Wario Bike, and races towards his enemies. If he falls off the bike, he will have to get back on it, as only one bike will appear on the screen. Wario can also throw the bike at an enemy, or vice versa. If the bike keeps being attacked, it will break into smaller parts. The bike parts can be picked up and thrown as well.